产品货号：
GS2156
中文名称：
Bsa29I内切酶
英文名称：
Bsa29I
产品规格：
500U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
使用说明
To obtain 100%activity,BSA should be added to the 1x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.
技术规格
相关搜索：Bsa29I内切酶，Bsa29I限制性内切酶
使用说明
To obtain 100%activity,BSA should be added to the 1x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA(dam-)in 1hourat 37℃ in a total reaction volume of 50μl. |
| Recognition sites | AT↑CGAT |
| 来源 | Bacillus stearothermophilus 29 |
| Assayed on | Lambda DNA(dam-) |
| Optimal buffer | G(10mMTris-HCl(pH 7.6 at 25℃);10mMMgCl2;50 mM NaCl;1 mM DTT.)+ BSA |
| Optimal temperature | 37℃ |
| Storage conditions | 10mMTris-HCl(pH 7.5);250 mM NaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;50%glycerol.Store at -20℃. |
| Ligations | After 20-fold overdigestion with enzyme more than 90%of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a.of enzyme for 16hoursat 37℃. |
| Methlation effect | Blocked by overlapping Dam methylation(GmATC):GATCGATC.|Blocked by CG methylation. |
| Thermal inactivation | enzyme is inactivated by incubation at 65℃ for 20 mins. |
| Campatible Ends | BanIII,BseCI,BshVI,BsiXI,Bsp106I,BspDI,BspXI,Bsu15I,BsuTUI,ClaI,ZhoI |
相关搜索：Bsa29I内切酶，Bsa29I限制性内切酶